Impact of Benzophenone UV Filter on Oxidative Stress Biomarkers and Antioxidant Defense in Labeo rohita
DOI:
https://doi.org/10.69980/ajpr.v28i1.137Keywords:
Oxidative stress, Acute Toxicity, Chronic toxicity, Ultraviolet Filters, Antioxidant enzymesAbstract
Lately, disintegration of ozone layer has headed to an upsurge in ultraviolet radiation, and its adverse effects on humans and other organisms are well known. As the ultraviolet (UV) filters are the chemical complexes used to protect against UV irradiation, the proliferating use of these filters has led toward an increase in environmental concentrations. Benzophenone (BP) has started to gain the attention of researchers due to its widespread use in cosmetics and personal care products and also its prevalence in the aquatic environment due to its low degradation and high accumulative potential. This study evaluated the 96-h acute toxicity and chronic effects of BP on oxidative stress biomarkers and antioxidant defense in Labeo rohita. Fish having the same weight were subjected to increasing concentration of BP (100-1000µg/L) for acute toxicity testing and their 96-h LC50 and lethal concentration were estimated as 606.67 µg/L and 1033.25 µg/L respectively. Oxidative stress in terms of superoxide dismutase (SOD) and catalase (CAT) was determined in the gills, liver, kidney, heart and muscles of L. rohita after 75 days chronic exposure of sub-lethal (1/3rd, 1/5th, and 1/7th of LC50) BP doses and sampling were done at 15, 30, 45, 60 and 75 days. A significant decline in CAT and SOD activity was observed during the exposure period showing excess reactive oxygen species (ROS) generation which is an indication of oxidative stress confirming the induced toxicity potential of BP.
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