Clinical Significance Of Salivary Siga, MMPS, Streptococcus Mutans and Streptococcus Sobrinus In Children With Dental Caries
DOI:
https://doi.org/10.69980/ajpr.v28i1.157Keywords:
Caries; Secretory immunoglobulin A; Matrix metalloproteinases; Streptococcus mutans; Streptococcus sobrinusAbstract
OBJECTIVE To investigate the clinical significance of secretory immunoglobulin A (sIgA), matrix metalloproteinases (MMPs), Streptococcus mutans and Streptococcus sobrinus in saliva of children with dental caries.
METHODS From August 2021 to May 2022, 120 children with severe caries diagnosed in our hospital's oral outpatient department were selected. They were divided into severe group (60 cases) and mild group (60 cases) according to the severity of the disease. In addition, 60 children without caries were selected as the control group. Oral examination was carried out for all children and caries loss compensation index was calculated; The levels of sIgA and MMPs in saliva were detected by ELISA, and the levels of Streptococcus mutans and Streptococcus sobrinus in saliva were detected by qRT PCR. The correlation between sIgA and MMPs and Streptococcus mutans and Streptococcus sobrinus was analyzed.
RESULTS (1) Caries loss and filling index: Compared with the control group, the caries loss and filling index of children in severe group and mild group increased significantly (P<0.05). (2) SIgA, MMP-2, MMP-9: Compared with the control group, the salivary sIgA, MMP-9 levels in the mild group were significantly higher (all P<0.05), and the salivary sIgA, MMP-2, MMP-9 levels in the severe group were significantly higher (all P<0.05); Compared with the mild group, the salivary sIgA and MMP-2 levels in the severe group were significantly higher (all P<0.05). (3) Streptococcus mutans and Streptococcus sobrinus: compared with the control group, the levels of Streptococcus mutans and Streptococcus sobrinus in saliva of mild group and severe group increased (P<0.05); Compared with the mild group, the levels of Streptococcus mutans and Streptococcus sobrinus in the saliva of the severe group were significantly higher (P<0.05). (4) Correlation analysis: The levels of Streptococcus mutans, Streptococcus sobrinus, sIgA, MMP-2, MMP-9 in saliva of children with dental caries were positively correlated with caries loss index (P<0.05). (5) Multivariate linear regression analysis: The levels of Streptococcus mutans, Streptococcus sobrinus, sIgA, MMP-2 and MMP-9 in saliva had a significant predictive effect on children with dental caries, and their increased levels would increase the risk of children with dental caries (P<0.05). CONCLUSIONS The increased levels of Streptococcus mutans, Streptococcus sobrinus, sIgA, MMP-2 and MMP-9 in saliva are related to the caries of children, and are positively related to the degree of caries.
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