Determination of Optimal Dietary Tryptophan Requirements for the Fingerlings of Labeo rohita, Cirrhinus mrigala, and Catla catla to Study Growth and Hematological Parameters under Stress.

Authors

  • Kaynat Saeed
  • Sajid Abdullah
  • Shakeela Parveen
  • Muhammad Shahbaz

DOI:

https://doi.org/10.69980/ajpr.v28i1.197

Keywords:

Tryptophan (TRP), Hematological Parameters, Growth performance, Temperature Stress

Abstract

Dietary tryptophan, an essential amino acid, enhances stress resilience, growth performance, and hematological health in fish under temperature fluctuations, negatively impacting their growth and physiological functions. This twelve-week study investigated the effects of dietary tryptophan (TRP) supplementation on growth performance and hematological parameters in three major carp species: Labeo rohita, Cirrhinus mrigala, and Catla catla. Fish were divided into a control group (TO, 0% TRP) and three experimental groups fed varying TRP levels: T1 (0.39%), T2 (1.43%), and T3 (1.12%). Fish were acclimated to two temperature regimes (16°C and 32°C), with 96 fingerlings per species in 12 glass aquaria (n=8 per aquarium). Results showed that TRP supplementation significantly enhanced hematological profiles in all species. At high TRP levels, L. rohita exhibited the highest RBC counts (1.027×10^6/µL) and hemoglobin levels (8.37g/dL), while C. mrigala had RBC counts of 1.173×10^6/µL and Hb of 8.50g/dL, and C. catla had the highest RBC counts (1.227×10^6/µL) and Hb (10.20g/dL). Growth performance improved markedly with TRP, particularly at 1.43% supplementation and 32°C: L. rohita displayed a superior feed conversion ratio while C. mrigala and C. catla showed the maximal growth responses under these conditions. Dietary tryptophan supplementation demonstrated a significant positive correlation with enhanced growth performance and restoration of hematological parameters in freshwater carp exposed to thermal stress. Compared to the control group, TRP supplementation led to statistically significant improvements (P < 0.05) in weight gain, specific growth rate (SGR), and feed conversion ratio (FCR). The 1.43% inclusion level proved most effective, offering optimal mitigation of temperature stress while maximizing growth enhancement.

Author Biographies

Kaynat Saeed

Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan

Sajid Abdullah

Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan

Shakeela Parveen

Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan

Muhammad Shahbaz

Department of Botany, University of Agriculture, Faisalabad, Pakistan

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2025-04-22

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Vol. 28 No. 1 (2025): American Journal of Psychiatric Rehabilitation

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