A Scanning Electron Microscopy Study of The Renal Glomeruli and Tubules in Adult Albino Rats Following Oral Administration of Sodium Fluoride, And the Possible Protective and Curative Role of Plant Leaf Extract Boerhaavia Diffusa L
DOI:
https://doi.org/10.69980/ajpr.v28i5.299Keywords:
Albino rats, Boerhaavia diffusa L., Scanning electron microscopy, Sodium fluorideAbstract
This study used microdissection methods, such as scanning electron microscopy, to clarify the morphological alterations in the kidneys of albino rats treated with sodium fluoride. Eight groups of six rats each were randomly selected from a total of forty-eight animals. Rats in Control I were given deionized water orally. For 40 days, rats in the first and second groups received daily doses of 300 and 600 mg NaF/kg bw, respectively. For 20 days, 500 mg/kg b.w./day of Boerhaavia diffusa L. leaf extract was given orally to Control II rats. After receiving a 20-day pretreatment of 500 mg/kg bw/day of Boerhaavia diffusa L. leaf extract, groups third and fourth were subjected to 40 days of exposure to 300 and 600 mg NaF/kg bw/ /day. Groups fifth and sixth were exposed firstly to 300 and 600 mg NaF/ kg bw /day and then post-treated with leaf extract of Boerhaavia diffusa L. for 20 days. Scanning electron microscopy was used to examine the morphological characteristics of the rat kidneys treated with sodium fluoride. The features included the shortening of the podocyte processes, cytoplasmic blebs, hypertrophy, atrophy, knob-like projections at the end of the podocytes, shrinkage of the cell body, extreme deprivation of erythrocytes, and collagen fibers. It might be said that sodium fluoride negatively impacted the kidney's morphological structure. The renal damage changes may be lessened by administering Boerhaavia diffusa L. leaf extract both before and after treatment.
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