Phytochemical Analysis, anti-inflammatory, analgesic and anti-spasmodic potential of Isodon regusus and Phytolacca latbenia
DOI:
https://doi.org/10.69980/ajpr.v28i1.504Keywords:
Isodon regusus and Phytolacca latbenia, phytochemical analysis, analgesic, antispasmodic and antiinflammtory.Abstract
Herbal medication from natural products has analgesic, antispasmodic and antiinflammtory effects on human health. This research aimed to investigate these major activities along with the phytochemical analysis. Phytochemical analysis was carried out using a series of standard qualitative tests following established protocol to detect the presence of various bioactive compounds. The analgesic activity was evaluated using the hot plate method, analgesic activity was performed using charcoal meal test and antiinflammtory activity was determined using carrageenan-induced paw edema model in rat. Qualitative Phytochemical screening of the ethanolic and methanolic extracts of I. rugosus and P. latbenia revealed the presence of key secondary metabolites including alkaloids, flavonoids, phenols and tannins. Quantitative analysis indicated that tannins are the most abundant phytochemical in I. rugosus with concentration of 79.41% in the ethanolic extract and 51.39% in the methanolic extract. Whereas, P. latbenia exhibited the highest concentration of alkaloids, 81.36% in the ethanolic extract and 73.70% in the methanolic extract. The analgesic potential of I. rugosus were assessed at doses of 100, 200 and 300mg/kg, where the ethanolic extract demonstrated a maximum decrease in latency time of (27.45%) followed by methanolic extract (24.30%) after 90minutes dose of 300mg/kg. Similarly the methanolic extract of P. latbenia showed the highest analgesic potential (51.94%) followed by methanolic extract with a 48.36% after 90 minutes at a dose of 300mg/kg. The antispasmodic activity of I. rugosus exhibited maximum percent inhibition of 68.34% in the ethanolic extract and 65.00% in the methanolic extract at a dose of 300mg/kg after 90minute. In contrast P. latbenia showed a maximum inhibition 51.74% in the ethanolic extract and 47.60% in the methanolic extract at same dose and interval of time. The anti-inflammatory activity of I. rugosus showed maximum percent decrease in paw volume (34.54%) in the ethanolic extract and (30.00%) in the methanolic extract after 90minute at a dose of 300mg/kg, while, P. latbenia exhibited high percent decrease in paw volume (40.59%) in the methanolic extract followed by ethanolic extract (28.71%) at a dose of 300mg/kg after 90 minutes. it was concluded that these both plants having the analgesic, antispasmodic and antiinflammtory potential due to the presence of various phytochemicals.
References
1. Adetunji, C. O., Egbuna, C., Oladosun, T. O., Akram, M., Micheal, O. S., Olisaka, F. N., ... & Olaniyan, O. T. (2021). Efficacy of Phytochemicals of Medicinal Plants for the Treatment of Human Echinococcosis: Echinococcal Disease, Hydatidosis, or Hydatid Disease Drug Discovery. Neglected Tropical Diseases and Phytochemicals in Drug Discovery, 225-243.Doi.org/10.1002/9781119617143.ch8
2. Akhlaq, M., Alum, M. K., & Alam, M. M. (2022). Anti-inflammatory potential of medicinal plants. Mediterranean Journal of Pharmacy and Pharmaceutical Sciences, 2(1), 15-23.
3. Akinmurele, O. J., Sonibare, M. A., Elujoba, A. A., Ogunlakin, A. D., Yeye, O. E., Gyebi, G. A., ... & Alanzi, A. R. (2023). Antispasmodic Effect of Alstonia boonei De Wild. and Its Constituents: Ex Vivo and In Silico Approaches. Molecules, 28(20), 7069. DOI.org/10.3390/molecules28207069
4. Asefa, M., Teshome, N., & Degu, A. (2022). Anti-inflammatory and analgesic activity of methanolic root extract of Verbascum sinaiticum benth. Journal of Inflammation Research, 6381-6392.
5. Ayanaw, M. A., Yesuf, J. S. & Birru, E. M. (2023). Evaluation of Analgesic and Anti-inflammatory Activities of Methanolic Leaf and Root Extracts of Gomphocarpus purpurascens A. Rich (Asclepiadaceae) in Mice. Journal of Experimental Pharmacology, 1-11.
6. Bhattacharya, A., Agrawal, D., Sahu, P. K., Kumar, S., Mishra, S. S., & Patnaik, S. (2014). Analgesic effect of ethanolic leaf extract of Moringa oleifera on albino mice. Indian journal of Pain, 28(2), 89-94.
7. Chaouche T, Haddouchi F, Bekkara FA (2011). Phytochemical study of root and leaves of the plant Echium pycmanthum Pomel. Der Pharm. Lett. 3(2):1-4
8. Chaulya, N.C., P.K. Haldar and A. Mukherjee. (2011). Antidiarrhoeal activity of methanol extract of the rhizomes of Cyperus tegetum Roxb. Int. J. Pharm. Pharm. Sci., 3(1): 133-135.
9. Chen, L., Deng, H., Cui, H., Fang, J., Zuo, Z., Deng, J., ... & Zhao, L. (2018). Inflammatory responses and inflammation-associated diseases in organs. Oncotarget, 9(6), 7204
10. Christy, A. O. (2023). In Vivo Evaluation of Analgesic Activities of Dioscorea dumetorum (Kunth) Pax and Tragia benthamii Bak. Sch Int J Tradit Complement Med, 6(2), 18-22.
11. Dahiru, D., Sini, J. M., & John-Africa, L. (2006). Antidiarrhoeal activity of Ziziphus mauritiana root extract in rodents. African journal of biotechnology, 5(10).
12. Dahiru, D., Sini, J. M., & John-Africa, L. (2006). Antidiarrhoeal activity of Ziziphus mauritiana root extract in rodents. African journal of biotechnology, 5(10).
13. Daniel, J. L., Vandi, P., Chessed, G., Umar, M., & Yusuf, A. I. (2024). Phytochemical Analysis of Medicinal Plants Used in the Treatment of Malaria Infection in Billiri and Funakaye Local Government Areas of Gombe State. BIMA JOURNAL OF SCIENCE AND TECHNOLOGY (2536-6041), 8(1B), 287-298.
14. Drossman, D. A. (2016). History of functional gastrointestinal symptoms and disorders and chronicle of the Rome Foundation. Rome IV functional gastrointestinal disorders: disorders of gut-brain interaction. Raleigh: Rome Foundation, Inc, 549-576.
15. Eddy, N.B. and D. Leimbach. 1953. Synthetic analgesics. II. Dithienylbutenyl- and dithienylbutylamines. J. Pharma. Exp. Therap., 107(3): 385–393.
16. Evans WC (2002). Pharmacognosy, 15th ed. English Language Book, Society Baillere Tindall: Oxford University Press.
17. Furman, D., Campisi, J., Verdin, E., Carrera-Bastos, P., Targ, S., Franceschi, C., ... & Slavich, G. M. (2019). Chronic inflammation in the etiology of disease across the life span. Nature medicine, 25(12), 1822-1832.
18. Gadamsetty, G., Maru, S., & Sarada, N. C. (2013). Antioxidant and Anti-inflammatory Activities of the Methanolic Leaf Extract of Traditionally Used Medicinal Plant Mimusops elengi L. Journal of pharmaceutical sciences and research, 5(6), 125.
19. Ghutke, T. D., Parvin, K., Rashida Banu, A. M., Bansal, S., Srivastava, A., Rout, S., & Ramzan, U. (2023). A comprehensive review on the therapeutic properties of medicinal plants. Acta Traditional Medicine. V2i01, 13-00.
20. Gupta, S. and A. Singh. 2017. Antimicrobial, analgesic and anti-inflammatory activity reported on Tamarindus indica Linn root extract. Pharmacog. J., 9(3): 410-416.
21. Harborne, J.B. (1998) Textbook of Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. 5th Edition, Chapman and Hall Ltd, London, 21-72.
22. Harborne, J.B. 1973. Photochemical methods: A guide to modern techniques of plant analysis.Chapman A. & Hall. London, pp: 22(9): 279.
23. Hossen, M. F., Nijhu, R. S., & Khatun, A. (2023). A phytochemical and pharmacological review on Dalbergia sissoo: A potential medicinal plant. Journal of Pharmacognosy and Phytochemistry, 12(1), 52-57.
24. Idu, M., Omoregbee, A., & Gabriel, B. O. (2023). Phytochemical, Antioxidant Screening, Antinociceptive, and Anti-inflammatory Activities of Boswellia dalzielii Hutch (Burseraceae) Root Ethanol Extract Using Animal Model. Biology, Medicine, & Natural Product Chemistry, 12(1), 143-150.
25. Islam, M. M., Pia, R. S., Sifath-E-Jahan, K., Chowdhury, J., Akter, F., Parvin, N., & Akter, S. (2013). Antidiarrheal activity of Dillenia indica bark extract. International Journal of Pharmaceutical Sciences and Research, 4(2), 682.
26. Ivan, K., Abdelgadir, A. A., & Crispin, S. D. (2023). Comparative Evaluation of the Anti-Inflammatory Properties of Methanol and Aqueous Crude Extracts of Apical leaves of Sida cuneifolia: An Ethnomedicinal Plant. Journal of Complementary and Alternative Medical Research, 24(4), 16-26.
27. Janbaz K.H., J. Arif, F. Saqib, I. Imran, M. Ashra, M. Zia-Ul-Haq, H.Z. Jaafar and V. De Feo (2014). In vitro and in-vivo validation of ethno pharmacological uses of methanol extract of Isodon rugosus Wall. ex Benth.(Lamiaceae). BMC Complement. Altern. Med 14(1): 71-82.
28. Khandelwal KR (2004). Practical Pharmacognosy, Techniques and experiments (12th Edn). Nirali Prakashan, Pune India. P 157.
29. Kokate, A., Li, X., & Jasti, B. (2008). HPLC detection of marker compounds during buccal permeation enhancement studies. Journal of pharmaceutical and biomedical analysis, 47(1), 190-194.
30. Kumar P, B. J. R., & Kiladi S, C. P. Preliminary Phytochemical and Pharmacognostic Studies of Holoptelea integrifolia Roxb. Ethnobotanical Leaflets, 2009 (10), 3.
31. Kumar, S., & Pandey, A. K. (2013). Chemistry and biological activities of flavonoids: an overview. The scientific world journal, 2013(1), 162750.Doi.org/10.1155/2013/162750
32. Li, Y., Kong, D., Fu, Y., Sussman, M. R., & Wu, H. (2020). The effect of developmental and environmental factors on secondary metabolites in medicinal plants. Plant Physiology and Biochemistry, 148, 80-89.
33. Liu, K. (2019). Effects of sample size, dry ashing temperature and duration on determination of ash content in algae and other biomass. Algal Research, 40, 101486.
34. Madaan, R., & Kumar, S. (2012). Screening of alkaloidal fraction of Conium maculatum L. aerial parts for analgesic and antiinflammatory activity. Indian journal of pharmaceutical sciences, 74(5), 457.DOI: 10.4103/0250-474X.108423
35. Madhu, M., Sailaja, V., Satyadev, T. N. V. S. S., & Satyanarayana, M. V. (2016). Quantitative phytochemical analysis of selected medicinal plant species by using various organic solvents. Journal of pharmacognosy and phytochemistry, 5(2), 25-29.
36. Mayouf, N., Charef, N., Saoudi, S., Baghiani, A., Khennouf, S., & Arrar, L. (2019). Antioxidant and anti-inflammatory effect of Asphodelus microcarpus methanolic extracts. Journal of ethnopharmacology, 239, 111914.
37. McCurdy, C. R., & Scully, S. S. (2005). Analgesic substances derived from natural products (natureceuticals). Life Sciences, 78(5), 476-484.
38. Mendoza, N., & Silva, E. M. E. (2018). Introduction to phytochemicals: secondary metabolites from plants with active principles for pharmacological importance. Phytochemicals: Source of antioxidants and role in disease prevention, 25, 1-5. DOI: 10.4103/0250-474X.108423
39. Mueller-Harvey, I., Bee, G., Dohme-Meier, F., Hoste, H., Karonen, M., Kölliker, R., & Waghorn, G. C. (2019). Benefits of condensed tannins in forage legumes fed to ruminants: Importance of structure, concentration, and diet composition. Crop Science, 59(3), 861-885. DOI: org/10.2135/cropsci2017.06.0369
40. Naeem, H., Gul, S., Khan, M., Hamid, S., Leghari, Q. A., Yasin, H., & Perveen, R. (2023). Anti-inflammatory and Analgesic Activity of Cleome brachycarpa Ethanolic Extract in Lower Mammals and Effects on Blood and Liver Enzymes. Jundishapur Journal of Natural Pharmaceutical Products.
41. Nawaz, H., Aslam, M., Shahzad, H., Mehboob, F., Waheed, R., Jabeen, R., ... & Ahmed, M. Z. (2022). Comparative Evaluation of Phytochemical Composition and Antioxidant potential of some Medicinally-important euphorbiaceous plants. japs: Journal of Animal & Plant Sciences, 32(6). DOI: org/10.36899/JAPS.2022.6.0578
42. Nitiema, M., Ouedraogo, W. R. C., Rainatou, B. O. L. Y., Ouedraogo, P. E., Noura, O. M., Kaboré, B., ... & Ouédraogo, S. (2023). Phytochemical profile, acute oral toxicity, antioxidant, and antispasmodic effects of ethyl acetate and aqueous residual fractions of Diospyros mespiliformis Hochst. ex A. DC (Ebenaceae) leaves on isolated duodenum of rat. Journal of Drug Delivery and Therapeutics, 13(12), 148-154.
43. Nunes, C. D. R., Barreto Arantes, M., Menezes de Faria Pereira, S., Leandro da Cruz, L., de Souza Passos, M., Pereira de Moraes, L., ... & Barros de Oliveira, D. (2020). Plants as sources of anti-inflammatory agents. Molecules, 25(16), 3726.
44. Oguntibeju, O. O. (2018). Medicinal plants with anti-inflammatory activities from selected countries and regions of Africa. Journal of inflammation research, 307-317.
45. Okoli, R. I., Turay, A. A., Mensah, J. K., & Aigbe, A. O. (2009). Phytochemical and antimicrobial properties of four herbs from Edo State, Nigeria. Report and opinion, 1(5), 67-73.
46. Okwuosa, C. N., E.N. Shu, N.C. Azubike, D.C. Nwachukwu, A.C. Onuba, I.N. Nubila, F.C. Otuu and I.J. Chukwu. 2016. Anti-diarrhoeal properties of the leaf extracts of Combretum racemosum. Beauv in rodents. Der. Pharma. Lett., 8 (18): 207-213.
47. Olalekan, M. A. (2023). Comparative Phytochemical and Antioxidant Analysis of the Leaf Extracts of Two Nigerian Medicinal Plants. Journal of Science and Mathematics Letters, 11(1), 30-38
48. Oliveira, I., Sousa, A., Ferreira, I. C., Bento, A., Estevinho, L., & Pereira, J. A. (2008). Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks. Food and chemical toxicology, 46(7), 2326-2331.
49. Ouahhoud, S., Marghich, M., Makrane, H., Karim, A., Khoulati, A., Mamri, S., ... & Saalaoui, E. (2023). In vitro assessment of myorelaxant and antispasmodic effects of stigmas, tepals, and leaves hydroethanolic extracts of Crocus sativus. Journal of Food Biochemistry, 2023.
50. Packirisamy, S., Gunam, V., Mahendra, J., Rajendran, D., & Rajagopal, P. (2023). Preliminary Phytochemical screening and Antioxidant properties of Methanolic root extract of Picrorhiza kurroa. Research Journal of Pharmacy and Technology, 16(9), 4266-4270.
51. Pearson, D. 1976. Chemical analysis of food.Churchill livington, Edinburgh, UK.103110 pp.
52. Pereira, R. B., Rahali, F. Z., Nehme, R., Falleh, H., Jemaa, M. B., Sellami, I. H., ... & Pereira, D. M. (2023). Anti-inflammatory activity of essential oils from Tunisian aromatic and medicinal plants and their major constituents in THP-1 macrophages. Food Research International, 167, 112678.
53. Peri, C and C. Pompei. 1971. Estimation of different phenolic groups in vegetable extracts. Phytochem., 10(9): 2187-2189.
54. Radovanović, K., Gavarić, N., & Aćimović, M. (2023). Anti-Inflammatory Properties of Plants from Serbian Traditional Medicine. Life, 13(4), 874.
55. Rauf, A., Jehan, N., Ahmad, Z., & Mubarak, M. S. (2017). Analgesic potential of extracts and derived natural products from medicinal plants. Pain Reli-From Analg to Altern Ther Rijeka: InTech, 339-51.
56. Revathi, M., Manonmani, R., & Sowmiya, V. (2024). PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF NELUBO NUCIFERA GAERTN. DOI: 10.20959/wjpr20247-31881
57. Sadiq, A., Zeb, A., Ullah, F., Ahmad, S., Ayaz, M., Rashid, U., & Muhammad, N. (2018). Chemical characterization, analgesic, antioxidant, and anticholinesterase potentials of essential oils from Isodon rugosus Wall. ex. Benth. Frontiers in pharmacology, 9, 623. DOI: Org/10.3389/fphar.2018.00623
58. Savithramma, N., Rao, M. L., & Suhrulatha, D. (2011). Screening of medicinal plants for secondary metabolites. Middle-East Journal of Scientific Research, 8(3), 579-584
59. Senguttuvan, J., Paulsamy, S., & Karthika, K. (2014). Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities. Asian Pacific journal of tropical biomedicine, 4, S359-S367.
60. Sharifullah, S. M., Khan, I., Ali, S., Ali, K., & Kumar, T. (2016). Study of important medicinal plants of district Dir Upper. J. Weed Sci. Res, 22(4), 595-606.
61. Shoaib, G., Shah, G. M., Hussain, M., Muhammad, S., Rehman, I. U., Khan, A., & Shah, M. (2017). Ethnomedicinal plants and traditional knowledge of some Phenorogams of lower Kaghan valley, district Mansehra, Pakistan. J. Appl. Environ. Biol. Sci, 7(5), 21-28.
62. Shoaib, G., Shah, G. M., Shad, N., Dogan, Y., Siddique, Z., Shah, A. H., & Nedelcheva, A. (2021). Traditional practices of the ethnoveterinary plants in the Kaghan Valley, Western Himalayas-Pakistan. Revista de Biología Tropical, 69(1), 1-11.
63. Sun, Y.-X., and J. -C. Liu (2011). Chemical constituents and biological activities of Euphorbia fischeriana STEUD. Chemistry and Iodiversity 8 (7): 1205–1214.
64. Sunil, M., Vedavijaya, T., Sayana, S. B., & Podila, K. S. (2023). Phytochemical Analysis and Antioxidant Evaluation of the Ethanolic Extract of the Leaves of Abutilon indicum. Cureus, 15(10).
65. Tadesse, E., E. Engidawork, T. Nedi and G. Mengistu. 2017. Evaluation of the anti-diarrheal activity of the aqueous stem extract of Lantana camara Linn (Verbenaceae) in mice. BMC compl. Alter. Med., 17(1): 190.
66. Ullah, N., Haq, I. U., Safdar, N., & Mirza, B. (2015). Physiological and biochemical mechanisms of allelopathy mediated by the allelochemical extracts of Phytolacca latbenia (Moq.) H. Walter. Toxicology and industrial health, 31(10), 931-937.
67. Winter, C.A and C.C. Poster. 1957. Effect of alteration in side chain up on anti-inflammatory and liver glycogen activities in hydrocortisone ester. J. American Pharmacol. Soc., 46:515-519.
68. Yasmeen, M., Prabhu, B., & Agashikar, N. (2010). Evaluation of the antidiarrhoeal activity of the leaves of Ixora coccinea Linn. in rats. Journal of clinical and diagnostic research, 4(5), 3298-3303.
69. Ybañez-Julca, R. O., Pino-Ríos, R., Quispe-Díaz, I. M., Asunción-Alvarez, D., Acuña-Tarrillo, E. E., Mantilla-Rodríguez, E., ... & Benites, J. (2023). Antispasmodic Effect of Valeriana pilosa Root Essential Oil and Potential Mechanisms of Action: Ex Vivo and In Silico Studies. Pharmaceutics, 15(8), 2072.
70. Zare, A., Khaksar, Z., Sobhani, Z., & Amini, M. (2018). Analgesic effect of Valerian root and turnip extracts. World Journal of Plastic Surgery, 7(3), 345. Doi: 10.29252/wjps.7.3.345
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